Pure antibodies 與 functional-grade antibodies 的區別

使用抗體對多種目標細胞進行抗原非依賴性和抗原特異性刺激是可靠且有效的激活和擴增原代細胞的方法，為了確保實驗結果的可靠性，Miltenyi Biotec 依據實驗應用，提供了 Pure antibodies 與 functional-grade antibodies 兩種類型的抗體。

Pure antibodies 與 functional-grade antibodies 的區別：

Pure antibodies

Pure functional-grade antibodies

抗體保存液中含有 stabilizer 和 0.05 % sodium azide

抗體經過無菌過濾處理並保存於不含防腐劑的 phosphate-buffered saline (PBS), pH 7.2

適用於 flow cytometry or immunofluorescence

1. 此類型抗體內毒素低，適用於 cell culture

2. 有兩類 Functional-grade antibodies : pure and biotin-conjugated。其中 Biotin-conjugated functional-grade antibodies 可以接到 Anti-Biotin MACSiBead Particles 去刺激活化細胞

熱門品項：

	order number	Name	clone	Size	Distribution of antigen:
Human	130-093-377	CD3-Biotin - functional grade, human	OKT3	100 µg in 1 mL	NKT cells, T cells, thymocytes
	130-093-387	CD3 pure - functional grade, human	OKT3	100 µg in 1 mL	NKT cells, T cells, thymocytes
	130-093-375	CD28 pure - functional grade, human	15E8	100 µg in 1 mL	B cells, lymphocytes, plasma cells, T cells, thymocytes
	130-094-133	CD40 pure - functional grade, human	HB14	100 µg in 1 mL	B cells, dendritic cells, endothelial cells, epithelial cells, fibroblasts, Langerhans cells, leukemia cells, lymphocytes, macrophages, mast cells, monocytes, plasma cells, platelets
	130-096-168	CD279 (PD1) pure - functional grade, human	PD1.3.1.3	500 µg in 0.5 mL	B cells, monocytes, spleen, T cells, thymocytes
	130-106-328	CD95 (FAS) pure - functional grade, human	DX2	500 µg in 0.5 mL	B cells, epithelial cells, fibroblasts, heart, kidney, liver, lung, monocytes, neutrophils, ovary, T cells, thymocytes
	130-098-855	CD282 (TLR2) pure - functional grade, human and mouse	REA109	500 µg in 0.5 mL	monocytes, granulocytes, dendritic, endothelial, and epithelial cells
	130-100-186	CD42b pure - functional grade, human	REA185	500 µg in 0.5 mL	platelets and megakaryocytes
	130-094-272	CD337 (NKp30) pure - functional grade, human	AF29-4D12	100 µg in 1 mL	NK cells
Mouse	130-093-182	CD28 pure - functional grade, mouse	37.51	100 µg in 1 mL	B cells, lymphocytes, plasma cells, T cells, thymocytes
	130-093-179	CD3ε-Biotin - functional grade, mouse	145-2C11	100 µg in 1 mL	NKT cells, T cells, thymocytes
	130-094-944	CD279 (PD1) pure - functional grade, mouse	HA2-7B1	500 µg in 1 mL	B cells, monocytes, spleen, T cells, thymocytes
	130-094-828	CD127 pure - functional grade, mouse	A7R 34	1 mg in 0.5 mL	B cells, bone marrow, liver, lymphocytes, monocytes, T cells, thymocytes
	130-093-022	CD40 pure - functional grade, mouse	FGK45.5	100 µg in 1 mL	B cells, dendritic cells, endothelial cells, epithelial cells, fibroblasts, Langerhans cells, leukemia cells, lymphocytes, macrophages, mast cells, monocytes, plasma cells, platelets
	130-093-023	CD40 pure - functional grade, mouse	FGK45.5	1 mg in 0.5 mL
	130-097-807	CD223 pure - functional grade, mouse	C9B7W	500 µg in 0.5 mL	NK cells, T cells
	130-098-856	CD252 (OX40L) pure - functional grade, mouse	RM134L	500 µg in 0.5 mL	B cells, dendritic cells, endothelial cells, heart, leukemia cells, mast cells, monocytes, spleen, T cells, thymocytes
	130-092-655	CD357 (GITR) pure - functional grade, mouse	DTA-1	100 µg in 1 mL	Activation of T cells upregulates CD357 expression. Interaction of CD357 with its ligand (GITRL) has been demonstrated to augment T cell activation, proliferation, cytokine production, as well as MAPKs and NF-κB activation.
	130-092-656	CD357 (GITR) pure - functional grade, mouse	DTA-1	1 mg in 0.5 mL
	130-091-978	CD317 (PDCA-1) pure - functional grade, mouse	JF05-1C2.4.1	1 mg in 0.5 mL	dendritic cells
	130-092-550	CD317 (PDCA-1) pure - functional grade, mouse	JF05-1C2.4.1	8 mg in 4 mL	dendritic cells

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