B Cell Expansion Kit, human

Links：https://www.miltenyibiotec.com/US-en/products/macs-cell-cult ...

The B Cell Expansion Kit has been developed for the activation and expansion of human B cells. The CD40-Ligand Multimer mimics a T cell-dependent activation of B cells. The B cell expansion is achieved by culturing and restimulation at day 7 and 10 of culture.

CD40-Ligand, also known as CD40L, CD154, TRAP, or TNFSF5 is a member of the TNF superfamily transiently expressed on the surface of activated CD4+ T lymphocytes. It is either expressed membrane-bound or proteolytically released as a soluble form which comprises 2/3 of the extracellular domain. Its receptor CD40 is found on antigen-presenting cells such as B cells, dendritic cells, and macrophages. CD40L-CD40 interaction is important in T cell-APC (antigen-presenting cell) interaction and is e.g. involved in B cell differentiation and proliferation, isotype class-switching, and protection of B cells from apoptosis. The B Cell Expansion Kit contains the CD40-Ligand and a cross-linking antibody for multimerization to increase the biological activity of CD40-Ligand, as well as premium grade Human IL-4 and StemMACS HSC Expansion Medium XF, human.

Applications: Activation and expansion of human B cells.

Order information:

Order no.	Product	Remarks
130-124-195	B Cell Expansion Kit, human - small size	2x Human CD40-Ligand Multimer Kit (130-098-775)
		Human IL-4, premium grade (130-093-919)
		StemMACS HSC Expansion Media XF, human (130-100-473)
130-106-196	B Cell Expansion Kit, human	2x Human CD40-Ligand Multimer Kit (130-098-776)
		Human IL-4, premium grade (130-093-919)
		StemMACS HSC Expansion Media XF, human (130-100-463)

Product data and images for B Cell Expansion Kit, human:

The components of the CD40-Ligand Multimer Kit were pre-incubated 30 minutes at room temperature. B cells were isolated using CD19 MicroBeads and cultured (StemMACS HSC Expansion Media XF supplemented with 5% AB serum and 2 µL IL-4) at a final density or 0.15×106 cells per mL (low density). On day 7 and 10 cells were harvested, counted, and restimulated. All cells were harvested, counted and analyzed on day 14. With the low density protocol a B cell expansion of up to 15-fold can be achieved.

Human B cell research Product tables:

Cell Type	Order no.	Product
Pan B cells	130-101-638	Pan B Cell Isolation Kit, human
	130-091-151	B Cell Isolation Kit II, human
	130-104-444	MACSxpress Whole Blood Pan B Cell Isolation Kit, human
B cell	130-050-301	CD19 MicroBeads, human
	130-097-055	CD19 MicroBeads, human - lyophilized
	130-091-104	CD20 MicroBeads, human
	130-046-401	CD22 MicroBeads, human
	130-055-301	CD19 MultiSort Kit, human
	130-117-034	REAlease CD19 MicroBead Kit, human
Naïve B cells	130-091-150	Naive B Cell Isolation Kit II, human
	130-091-333	CD43 MicroBeads, human
	130-103-775	Anti-IgD MicroBeads, human
Plasmablasts	B cell isolation kit II + CD38 Microbeads
	130-091-151	B Cell Isolation Kit II, human
	130-092-263	CD38 MicroBead Kit, human
Plasma cells	130-051-301	CD138 MicroBeads, human
	130-097-614	CD138 MicroBeads, human - lyophilized
	130-093-628	Plasma Cell Isolation Kit II, human
Memory B cells	130-093-546	Memory B Cell Isolation Kit, human
	130-093-619	IgM+ Memory B Cell Isolation Kit, human
	130-094-350	IgG+ Memory B Cell Isolation Kit, human
	130-093-617	Switched Memory B Cell Isolation Kit, human
	130-051-601	CD27 MicroBeads, human
	130-047-501	Anti-IgG MicroBeads, human
	130-093-230	Anti-IgM MicroBeads, human
Abnormal B cells	130-103-929	Anti-ROR1 MicroBead Kit, human
	130-051-301	CD138 MicroBeads, human
	130-097-614	CD138 MicroBeads, human - lyophilized
	130-103-466	B-CLL Cell Isolation Kit, human
	130-093-452	CD10 MicroBead Kit, human

The complete portfolio for your B cell research

A standardized and reproducible method for expansion and activation of human primary B cells

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