Links:https://www.miltenyibiotec.com/US-en/applications/by-researc ...
TumorMACS Media are optimized, standardized, and serum-free media for the reliable expansion of tumor cells from primary or xenotransplanted human tumor samples. Taking into account that different tumor entities have specific culture requirements, Miltenyi Biotec has developed tumor-specific TumorMACS Supplements. Accordingly, the appropriate specific medium should be used to achieve optimal cell culture results for a particular tumor entity. Tumor cells from freshly dissociated tissue can be cultured over multiple passages, thus allowing for in vitro assays, such as drug screenings on primary cell cultures or characterization studies including gene expression or protein profiling. In addition, TumorMACS Media support the establishment of new cell lines and the generation of cell numbers that are sufficiently large for further experiments, such as in vivo transplantation studies in animals. TumorMACS Media efficiently expand cultures of primary tumor cells in vitro while maintaining the cell populations’ heterogeneity and tumorigenic potential. The media are manufactured under controlled conditions using ingredients of high quality. TumorMACS Media offer consistent lot-to-lot performance and optimal conditions for the cultivation of primary tumor cells.
Cell lines derived from primary tumors and propagated in Renal TumorMACS Medium retained their initial heterogeneity and closely and reliably resemble the initial patient tumor.
Order information:
Miltenyi Biotec products | Order no. |
Tissue storage | |
MACS® Tissue Storage Solution | 130-100-008 |
Tumor dissociation | |
Tumor Dissociation Kit, human | 130-095-929 |
C Tubes | 130-096-334 |
gentleMACS™ Octo Dissociator with Heaters | 130-096-427 |
MACS SmartStrainers (30 µm) | 130-110-915 |
MACS SmartStrainers (70 µm) | 130-110-916 |
MACS SmartStrainers (100 µm) | 130-110-917 |
Tumor cell isolation | |
Tumor Cell Isolation Kit, human | 130-108-339 |
Mouse Cell Depletion Kit | 130-104-694 |
Flow cytometry | |
MACS Antibodies, including REAfinity™ Recombinant Antibodies | |
Cell Culture | |
Pancreas TumorMACS Medium | 130-119-484 |
Ovarian TumorMACS Medium | 130-119-483 |
Renal TumorMACS Medium | 130-119-482 |
Y-27632 (ROCK inhibitor) | 130-104-169 |
Cryopreservation | |
StemMACS™ Cryo-Brew | 130-109-558 |