科學界正在全力以赴與嚴重的急性呼吸系統綜合症冠狀病毒 2（SARS‑CoV‑2）作對抗。 Miltenyi Biotec 致力於協助這些研究人員深入了解 SARS‑CoV‑2。

我們新開發的 SARS-CoV-2 PepTivator 胜肽庫可用於加載抗原呈遞細胞和刺激 SARS-CoV-2 特異性 T 細胞。然後可以檢測和/或分離 T 細胞用於進一步研究。我們提供了四個不同的胜肽庫，涵蓋了三種主要結構性 SARS-CoV-2 蛋白的序列。

SARS‑CoV‑2 PepTivator 胜肽庫的應用數據範例

1. 恢復的 COVID-19 患者中 SARS-CoV-2 特異性 T 細胞的檢測
   恢復的 COVID-19 患者在 SARS‑CoV‑2 PepTivator 胜肽庫刺激下產生大量釋放 IFN-γ 的 T 細胞。
   Recovered COVID-19 patients show elevated levels of IFN-γ–producing T cells upon stimulation with SARS-CoV-2 PepTivator Peptide Pools
   
   Samples of a healthy donor and a recovered COVID-19 patient were stimulated for 4 h with the indicated SARS-CoV-2 PepTivator Peptide Pools (Prot_N, Prot_M, Prot_S, or a mix of all three) in the presence of BFA. As negative control, samples were left untreated without (w/o) antigen. Subsequently, T cell lineage surface markers and intracellular cytokines were stained. The presented plots are exemplary data showing IFN-γ+CD3+ T cells. The data demonstrate an increased number of activated T cells in recovered COVID-19 patients upon stimulation with the SARS-CoV-2 peptide pools. Quantitative analysis is shown below.
   Unless otherwise specifically indicated, Miltenyi Biotec products and services are for research use only and not for therapeutic or diagnostic use.
   
   
2. SARS‑CoV‑2 特異性 T 細胞的定量分析
   Exemplary quantitative analysis of SARS-CoV-2–specific T cells
   
   Samples of a healthy donor and a recovered COVID-19 patient were stimulated with SARS-CoV-2 PepTivator Peptide Pools (Prot_N, Prot_M, Prot_S, or a mix of all three) and examined for IFN-γ production. Stained cells were analyzed using flow cytometry and the frequencies of IFN-γ+CD3+ T cells are shown in the bar chart. Flow data are shown above.
   Unless otherwise specifically indicated, Miltenyi Biotec products and services are for research use only and not for therapeutic or diagnostic use.
   
   
3. SARS-CoV-2 特異性 T 細胞的富集
   可以使用快速抗原特異性 T 細胞富集（Rapid ARTE）來富集罕見的 SARS‑CoV‑2 特異性 T 細胞。樣品用 SARS‑CoV‑2 混合 PepTivator 刺激，染色（CD3，CD4，CD8，CD154和TNF-α），並採用 Rapid ARTE 方案富集。
   Exemplary enrichment of SARS-CoV-2–specific T cells
   
   Samples of a healthy donor and a recovered COVID-19 patient were stimulated with SARS-CoV-2 PepTivator Peptide Pools (mix of Prot_N, Prot_M and Prot_S), left untreated without (w/o) antigens as negative control, or incubated with influenza antigens as positive control. Based on the Rapid ARTE protocol, the cells were subsequently stained (CD3, CD4, CD8, CD154, and TNF-α) and enriched. Cells were gated on CD4+ cells for the presented exemplary flow data. Analysis of CD154 and TNF-α shows successful enrichment of rare SARS-CoV-2–specific CD4+ T cells.
   Data are kindly provided by Alexander Scheffold and Petra Bacher, UKSH, Kiel, Germany.
   Unless otherwise specifically indicated, Miltenyi Biotec products and services are for research use only and not for therapeutic or diagnostic use.

SARS‑CoV‑2 PepTivator® Peptide Pools 產品資訊：