元昕實業有限公司

Miltenyi Biotec 開發了用於激活和擴增人類 B 細胞的 B 細胞擴增試劑盒。利用 CD40- 配體多聚體 (CD40-Ligand Multimer) 模擬T細胞依賴的B細胞活化，並在培養的第 7 天和第 10 天進行再刺激來實現 B 細胞的擴增。
CD40- 配體 (CD40-Ligand)，也稱為 CD40L、CD154、TRAP 或 TNFSF5，是在活化的 CD4+ T 淋巴細胞表面瞬時表達的 TNF 超家族的成員。它以膜結合的形式表達或以蛋白水解形式釋放為包含 2/3 細胞外結構域的可溶性形式。在抗原呈遞細胞（例如 B 細胞，樹突狀細胞和巨噬細胞）上發現了其受體 CD40 receptor。CD40L-CD40 相互作用在 T 細胞 -APC（抗原呈遞細胞）相互作用中很重要，並且例如是 CD40L-CD40 相互作用。參與 B 細胞的分化和增殖，同種型類別轉換以及 B 細胞免於凋亡的保護作用。

產品資訊：

貨號	品項	內容
130-124-195	B Cell Expansion Kit, human - small size	2x Human CD40-Ligand Multimer Kit (130-098-775)
		Human IL-4, premium grade (130-093-919)
		StemMACS HSC Expansion Media XF, human (130-100-473)
130-106-196	B Cell Expansion Kit, human	2x Human CD40-Ligand Multimer Kit (130-098-776)
		Human IL-4, premium grade (130-093-919)
		StemMACS HSC Expansion Media XF, human (130-100-463)

原廠測試數據：

The components of the CD40-Ligand Multimer Kit were pre-incubated 30 minutes at room temperature. B cells were isolated using CD19 MicroBeads and cultured (StemMACS HSC Expansion Media XF supplemented with 5% AB serum and 2 µL IL-4) at a final density or 0.15×106 cells per mL (low density). On day 7 and 10 cells were harvested, counted, and restimulated. All cells were harvested, counted and analyzed on day 14. With the low density protocol a B cell expansion of up to 15-fold can be achieved.

各類型 B 細胞分選試劑：

細胞類型	貨號	品項	說明
Pan B cells	130-101-638	Pan B Cell Isolation Kit, human	透過monoclonal antibodies against CD2, CD3, CD4, CD14, CD15, CD16, CD34, CD56, CD61, CD235a (Glycophorin A) and FceRIa移除非目標細胞
	130-091-151	B Cell Isolation Kit II, human	透過monoclonal antibodies against CD2, CD14, CD16, CD36, CD43, and CD235a (Glycophorin A)移除非目標細胞
	130-104-444	MACSxpress Whole Blood Pan B Cell Isolation Kit, human
B cell	130-050-301	CD19 MicroBeads, human
	130-097-055	CD19 MicroBeads, human - lyophilized
	130-091-104	CD20 MicroBeads, human
	130-046-401	CD22 MicroBeads, human
	130-055-301	CD19 MultiSort Kit, human	可移除標記於細胞上的磁珠
	130-117-034	REAlease CD19 MicroBead Kit, human	可移除標記於細胞上的磁珠
Naïve B cells	130-091-150	Naive B Cell Isolation Kit II, human
	130-091-333	CD43 MicroBeads, human
	130-103-775	Anti-IgD MicroBeads, human
Plasmablasts	B cell isolation kit II + CD38 Microbeads
	130-091-151	B Cell Isolation Kit II, human
	130-092-263	CD38 MicroBead Kit, human
Plasma cells	130-051-301	CD138 MicroBeads, human
	130-097-614	CD138 MicroBeads, human - lyophilized
	130-093-628	Plasma Cell Isolation Kit II, human
Memory B cells	130-093-546	Memory B Cell Isolation Kit, human
	130-093-619	IgM+ Memory B Cell Isolation Kit, human
	130-094-350	IgG+ Memory B Cell Isolation Kit, human
	130-093-617	Switched Memory B Cell Isolation Kit, human
	130-051-601	CD27 MicroBeads, human
	130-047-501	Anti-IgG MicroBeads, human
	130-093-230	Anti-IgM MicroBeads, human
Abnormal B cells	130-103-929	Anti-ROR1 MicroBead Kit, human
	130-051-301	CD138 MicroBeads, human
	130-097-614	CD138 MicroBeads, human - lyophilized
	130-103-466	B-CLL Cell Isolation Kit, human
	130-093-452	CD10 MicroBead Kit, human

The complete portfolio for your B cell research

A standardized and reproducible method for expansion and activation of human primary B cells

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