UltraMicroscope Blaze™
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Automated light sheet imaging of large samples
Discover our fully automated light sheet microscope UltraMicroscope Blaze for imaging multiple or very large samples with subcellular resolution. Explore microscopy at a different level to accelerate your projects and pave the way for new insights. The combination of our pioneering UltraMicroscope technology with the latest developments in the field of light sheet optics and sample preparation guarantees best data quality.
UltraMicroscope Blaze™ is the only fully automated light sheet microscope for imaging large or multiple cleared samples at subcellular resolution. Our pioneering UltraMicroscope technology combined with cutting-edge light sheet optics ensures excellent data quality.
A standard sample chamber allows convenient imaging of multiple rodent organs or organoids. Additionally, the new XXL chamber represents a powerful upgrade, specially designed to provide the largest possible sample space for the UltraMicroscope Blaze. It enables fitting of samples as big as a human kidney or whole adult mouse models.
Full automation
The UltraMicroscope Blaze enables an automated sample release and smooth switching between different objectives and magnification lenses, while keeping images sharp with the autofocus feature.
Large sample chambers
A standard sample chamber allows convenient imaging of multiple rodent organs or organoids, and the new XXL chamber provides the largest possible sample space for the UltraMicroscope Blaze.
Easy adaptation
Whether imaging large organs or even entire mouse models, our wide range of dipping caps guarantees high image quality in all clearing media - from water to organic solvents.
High-resolution
The combination of the MI Plan objective lenses and the cutting-edge light sheet illumination technology ensures unprecedented image quality from overview to sub-cellular details.
Light sheet technology tailored to your sample
As the generated light sheet diverges, it approximates a plane only over a given horizontal range. This is where the light sheet is thinnest and where fluorescence detection takes place. To achieve an appropriate illumination for a particular sample, the planar range of the light sheet has to be matched with the sample size and the desired field of view (FOV). The light sheet of the UltraMicroscope Blaze is tailored to the sample size by using adjustable light sheet parameters.
A low numerical aperture (NA) of illumination results in a broad FOV at the expense of a low Z-resolution when imaging large samples (A). In contrast, a high NA results in a high Z-resolution and a narrow FOV suitable for imaging small samples (B), with a full range of gradations in between. Where both high Z-resolution and a large FOV are essential, for example when imaging larger samples, the UltraMicroscope Blaze offers dynamic horizontal focusing. To that end, a sequential series of high-resolution images are taken across the desired FOV and automatically stitched together into a single high-quality image by our ImSpector image acquisition software (C).
UltraMicroscope Blaze™ applications in immuno-oncology research
- Visualization of single disseminated cancer cells in whole animal models
- Drug target identification for cancer treatments in a whole mouse body or entire organs
- Section-free 3D histological analysis
In the study published in Cell, the group of Ali Ertürk from the Institute for Tissue Engineering and Regenerative Medicine (iTERM), Helmholtz Zentrum München, Germany used the UltraMicroscope Blaze to identify hundreds of small metastases throughout the mouse body.
According to the researchers, this method could significantly increase the success rate of cancer drug candidates.