In the biopharmaceutical industry, many biological drugs are produced in mammalian cells. Cell line development (CLD) is used to determine which cell lines have the highest recombinant protein production and are also stable during large-scale manufacturing.

In the CLD workflow, cell lines undergo incremental scaling of culture from static to shaker flask expansion and further scaling up in bioreactors. Cell culture in early CLD is currently limited to static format as the size of the well plates and number of cells limit the ability to agitate the culture.

CYTENA Bioprocess Solutions has developed their new microbioreactor to disrupt the field of cell line development (CLD).

The C.BIRD™ device consists of an autonomous control system and a consumable C.BIRD™ lid for mixing culture media and cells.

The S.NEST™ increases efficiency and productivity for biologics production, drug screening and functional genomics.

Microbioreactors have been instrumental in a string of research breakthroughs in CLD for a range of downstream applications, but with the addition of real-time monitoring, the S.NEST is at the forefront of those advances. Furthermore, it boasts an impressive merger of enhanced features, including patented technology for reciprocating mixing, incubation chambers with individual environmental controls, a camera module for real-time pH and dissolved oxygen monitoring, powerful analytical software to crunch data, compatibility with 96-well or 24-well culture plates, and four chambers for the high-throughput culturing of as many as 384 wells at once.

The C.NEST™ designed to support superior cell growth and maintenance of a variety of cell lines and mammalian cells, even in single-cell and 3D cultures. The chamber’s height allows two microwell plates to be placed in one chamber.

Please kindly refer to application note: https://www.cytena-bps.com/application-note/

TRADITIONAL WORKFLOW

CYTENA WORKFLOW

CYTENA BPS portfolio:

C.BIRD Flyer

S.NEST Flyer

C.NEST Flyer