Extraction and magnetic isolation of cell organelles and exosomes

Our isolation kits based on MACS® Technology use nano-sized super-paramagnetic MACS MicroBeads and enable high-yield isolation of pure and functional organelles and exosomes in as little as two hours. Our technology allows for minimal labeling, thus ensuring the preservation of the organelle natural state, for higher quality and more reliable downstream analysis.

1. Mitochondria isolation and extraction - Gentle isolation of pure and functional mitochondria with high yields in less than 2 hours
   
   Tissue homogenates generated with the Mitochondria Extraction Kit are immediately ready for magnetic isolation of mitochondria. Our Mitochondria Isolation Kits for human and mouse samples use Anti-TOM22 MicroBeads to enable the isolation of pure and functional mitochondria with high yields in less than two hours. Mitochondria isolated with our kits, are suitable for downstream functional assays.
   
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   	Order no.	Product	Size	Remarks
   Mitochondria Extraction	130-097-340	Mitochondria Extraction Kit - Tissue	for 25  preparations	Use with the gentleMACS Dissociators and C-tube to accelerate and simplify the isolation of mitochondria from human and mouse tissue.
   	Additional requirements
   	04 693 159 001 (Merck)	cOmplete™, Mini, EDTA-free Protease Inhibitor Cocktail Tablets provided in EASYpacks	30 TABLETS
   	130-095-823	Pre-Separation Filters, 70 µm	50 test
   	130-093-237	gentleMACS C Tubes	25 test
   Mitochondria Isolation	130-094-532	Mitochondria Isolation Kit, human	for 25  preparations	Contains 1.25 mL Anti-TOM22 MicroBeads, human/25 mL Lysis Buffer/56.25 mL 10× Separation Buffer/27.50 mL Storage Buffer/25 LS Columns
   	130-096-946	Mitochondria Isolation Kit, mouse tissue	for 25  preparations	Contains 1.25 mL Anti-TOM22 MicroBeads, mouse/25 mL Lysis Buffer/56.25 mL 10× Separation Buffer/27.50 mL Storage Buffer/25 LS Columns/25 Pre-Separation Filters (30 µm)
   	130-097-039	Mitochondria MidiMACS™ Starting Kit, mouse tissue	1KIT	Contains Mitochondria Isolation Kit, mouse tissue (130-096-946)/MidiMACS™ Separator (130-042-302)/MACS® MultiStand (130-042-303)
   	130-097-040	Mitochondria QuadroMACS™ Starting Kit, mouse tissue	1KIT	Contains Mitochondria Isolation Kit, mouse tissue (130-096-946)/QuadroMACS™ Separator (130-090-976)/MACS® MultiStand (130-042-303)
   	130-127-693	Anti-TOM22 MicroBeads, mouse	for 25  preparations	1.25 mL Anti-TOM22 MicroBeads, mouse

   
   
   
2. Exosome isolation - Targeted isolation of CD9+, CD63+, or CD81+ extracellular vesicles from cell culture supernatant or body fluids in less than two hours
   
   High yield isolation of pure exosomes or extracellular vesicles (EVs) directly from cell culture supernatant, plasma, urine, or ascites. Our Exosome Isolation Kits for human and mouse samples enable fast and convenient isolation of intact exosomes or EVs by positive selection using magnetic MicroBeads. Find out more about our research solutions for exosome isolation and analysis.
   
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   	Order no.	Product	Size	Remarks
   Exosome Isolation (human)	130-110-912	Exosome Isolation Kit Pan, human	for 20  isolations	1 mL Exosome Isolation MicroBeads Pan,human (Cocktail of MicroBeads conjugated to CD9, CD63, and CD81)/4 mL Equilibration Buffer/25 mL Isolation Buffer/20 µ Columns and plungers
   	130-110-913	Exosome Isolation Kit CD9, human	for 20  isolations	Exosome Isolation Kit CD9, human/4 mL Equilibration Buffer/25 mL Isolation Buffer/20 µ Columns and plungers
   	130-110-914	Exosome Isolation Kit CD81, human	for 20  isolations	Exosome Isolation Kit CD81, human/4 mL Equilibration Buffer/25 mL Isolation Buffer/20 µ Columns and plungers
   	130-110-918	Exosome Isolation Kit CD63, human	for 20  isolations	Exosome Isolation Kit CD63, human/4 mL Equilibration Buffer/25 mL Isolation Buffer/20 µ Columns and plungers
   	130-111-572	Exosome Starting Kit Pan, human	1 KIT	Contains Multistand
   	130-111-573	Exosome Starting Kit CD9, human	1 KIT	Contains Multistand
   	130-111-576	Exosome Starting Kit CD63, huma	1 KIT	Contains Multistand
   	130-111-575	Exosome Starting Kit CD81, human	1 KIT	Contains Multistand
   Exosome Isolation (mouse)	130-117-040	Exosome Isolation Kit CD81, mouse	for 20  isolations	Exosome Isolation Kit CD81, mouse/4 mL Equilibration Buffer/25 mL Isolation Buffer/20 µ Columns and plungers
   	130-117-041	Exosome Isolation Kit CD63, mouse	for 20  isolations	Exosome Isolation Kit CD63, mouse/4 mL Equilibration Buffer/25 mL Isolation Buffer/20 µ Columns and plungers
   	130-117-042	Exosome Isolation Kit CD9, mouse	for 20  isolations	Exosome Isolation Kit CD9, mouse/4 mL Equilibration Buffer/25 mL Isolation Buffer/20 µ Columns and plungers
   	130-117-039	Exosome Isolation Kit Pan, mouse	for 20  isolations	1 mL Exosome Isolation MicroBeads Pan,mouse (Cocktail of MicroBeads conjugated to CD9, CD63, and CD81)/4 mL Equilibration Buffer/25 mL Isolation Buffer/20 µ Columns and plungers
   	130-118-915	Exosome Starting Kit CD63, mouse	1 KIT	Contains Multistand
   	130-118-916	Exosome Starting Kit CD81, mouse	1 KIT	Contains Multistand
   	130-118-917	Exosome Starting Kit CD9, mouse	1 KIT	Contains Multistand
   	130-118-918	Exosome Starting Kit Pan, mouse	1 KIT	Contains Multistand
   Multiplex assays	130-122-209	MACSPlex Exosome Kit, human	for up to 24 tests	Allow detection of 37 exosomal surface epitopes plus two isotype controls
   	130-108-813	MACSPlex Exosome Kit, human	for up to 96 tests	Allow detection of 37 exosomal surface epitopes plus two isotype controls

   
   
   
3. Nuclei extraction - Gentle and effective generation of single nuclei suspensions from fresh and frozen tissue samples in only 7 minutes
   
   The Nuclei Extraction Buffer is designed to gently, rapidly, and effectively generate single nuclei suspensions from fresh and frozen tissues. It has been developed for use with the gentleMACS Dissociators and C Tubes.