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Recombinant antibodies for mass cytometry
- Large portfolio of recombinant clones for consistent conjugation performance and reproducible results
- Provided in a stabilizer-free aqueous buffer suitable for conjugation
- Conjugation-optimized concentration of 1 µg/µL
Mass cytometry
Mass cytometry is a method that combines flow cytometry with inductively coupled plasma mass spectrometry (ICP-MS). The fusion of these two technologies facilitates the simultaneous analysis of over 40 cellular parameters on a single-cell level, enabling the investigation of complex cellular systems. The usage of metal labeling instead of fluorochome conjugation, as used for conventional flow cytometry, results in none to minimal spectral overlap.
As a high-parameter technique, mass cytometry depends on standardized antibodies that ensure consistent performance. To improve your data quality when it comes to mass cytometry, we have extended our portfolio to include recombinantly engineered antibodies specifically optimized for CyTOF® applications.
As a high-parameter technique, mass cytometry depends on standardized antibodies that ensure consistent performance. To improve your data quality when it comes to mass cytometry, we have extended our portfolio to include recombinantly engineered antibodies specifically optimized for CyTOF® applications.
Why use recombinant antibodies?
Our REAfinity Recombinant Antibodies provide high lot-to-lot consistency, resulting in reliable and reproducible results. Advantages for mass cytometry users include:
Recombinantly generated
Highly reproducible results through consistent antibody quality
Mutated Fc region abolishing FcγR binding
Made to eliminate background signal
Consistent human IgG1 isotype
No isotype-dependent variation during metal conjugation
Expression in standardized mammalian culture system
No unwanted Ig impurities
High lot-to-lot consistency
Accurate comparison of flow cytometry and mass cytometry experiments with the same REAfinity Antibody clones
Highly reproducible results through consistent antibody quality
Mutated Fc region abolishing FcγR binding
Made to eliminate background signal
Consistent human IgG1 isotype
No isotype-dependent variation during metal conjugation
Expression in standardized mammalian culture system
No unwanted Ig impurities
High lot-to-lot consistency
Accurate comparison of flow cytometry and mass cytometry experiments with the same REAfinity Antibody clones
Figure 1: Advantages of REAfinity Recombinant Antibodies.
REAfinity Anitbodies have been optimized for flow cytometry applications and are therefore easily transferrable to your CyTOF® application
REAfinity Anitbodies have been optimized for flow cytometry applications and are therefore easily transferrable to your CyTOF® application
Comparison between flow and mass cytometry. (A) Human peripheral blood mononuclear cells (PBMCs) were stained with CD159c (NKG2C)-PE (REA205) and analyzed using flow cytometry. (B) The same sample was stained with the metal-conjugated pure version of CD159c (NKG2C) (REA205) and analyzed using mass cytometry. Data courtesy of Prof. Karl Johan Malmberg, Oslo University Hospital, Norway.
Performance data:
"We have tested most commercially available clones for NK cell markers and found that REAfinity Recombinant Antibodies perform far superior compared to traditional monoclonal antibodies, resulting in a much better resolution of our target cells in mass cytometry experiments."
Brochure
REAfinity™ Antibodies for mass cytometry – Recombinantly engineered to increase reproducibility
Application note
Extensive characterization of NK cells using mass cytometry