StemMACS™ DiffBase XF

StemMACS™ DiffBase XF (xeno-free) is an optimized and standardized base medium for the differentiation of human pluripotent stem cells (hPSCs). StemMACS DiffBase XF has been developed to be used directly on hPSCs cultivated in StemMACS iPS-Brew XF and, when supplemented with the appropriate patterning cytokines and small molecules, can be used as a base medium for virtually every type of directed differentiation protocol. Moreover, without the need for further cytokines supplementation, StemMACS DiffBase XF can be conveniently used as media to assess hPSCs pluripotency by embryoid body assay.

Suitable for hPSCs-pluripotency assessment by embryoid bodies assay. StemMACS™ DiffBase XF supports cell survival and multiplication also without cytokines addition and can be therefore additionally used as media to assess PSC pluripotency by embryoid body assay. In fact, StemMACS DiffBase XF supports the formation and long-term survival of embryoid bodies (EBs, fig. 3).EBs grown in suspension for two weeks in StemMACS DiffBase XF and for 1 week in adherent culture, show differentiation in cellular types deriving from the 3 embryonic germ layers.

Applications

• Directed differentiation of hPSCs as monolayer adherent cultures or via embryoid bodies formation (3D differentiation).
• Pluripotency assessment by embryoid bodies assay (spontaneous differentiation)