Celetrix Electroporation

Celetrix Adavantage

1. Creatively grouping all mammalian cell types into two groups, each group only needs voltage micro-adjustment. Extremely simple operation;
2. Great efficiency and cell viability, even for large plasmids;
3. Low cost:
   - low cost kits;
   - universal kits, no need to stock up for different cells;
   - smooth experiments, less kits used.
4. Honest concepts, no hoaxes such as “direct transfection of nucleus” and “different buffer for different cells”. Fast technical support solving Users’ electroporation problems.
   
   
   

Limitation of cuvettes

1. Within the cuvette, the upper portion is a poor area for electroporation as well as the two side portions adjacent to the electrodes.
2. After cutting out the poor areas, the real optimal area (shown as the blue block at the right) in the cuvette is small. For 1mm and 2mm cuvettes, there might be no optimal area at all since the bubbles can spread from the electrodes quite vigorously.

Celetrix Electroporation - New Era for T Cell Immunotherapy

Primary cells transfection

The Celetrix electroporation technology can electroporate primary cells at very high efficiency. For example, Human PBMC transfection efficiency with GFP plasmid is 64%, and transfection efficiency of CAR-T2A-GFP with SB transposon can reach 60-90%.Human iPSC can be electroporated at a very high efficiency of more than 80% and the cells remain undifferentiated. The Celetrix efficiency is highest compared to all other electroporation methods on the market.

Genome editing by CRISPR/Cas9

With Celetrix electroporation, we can deliver RNP (ribonucleoprotein complex containing Cas9 protein and gRNA) or Cas9/gRNA plasmid into cells to perform gene editing.
The Celetrix™ technology can electroporate human iPSC with Cas9/gRNA. The average efficiency on 4 targeting genes (APP, AAVS1, OCT4 and PDCD1) is more than 50%, and the targeting efficiency on AAVS1 site can be 70%.

 User friendly system:

The eletroporator uses our proprietary electroporation tubes and adapters are used to accommdate different tube sizes.

1. Plug in power cable and switch on the machine. The eletroporator will run a self-test and then proceed to the user interface.
2. Voltage/ Pulse time/ Pulse no./ Pulse interval can be set. We use a single pulse at 30 milliseconds for most cell lines. You'll only need to adjust the voltage. 
3. Centrifuge the cells to a pellet and wash the cells once with a serum-free medium.
4. During centrifugation, the electroporation buffer can be prepared. (Celetrix electroporation buffer has 2 components: Part A and Part B. Freshly mix them at 1:1 ratio.)
5. Resuspend the washed cells with the electroporation buffer.
6. Add the plasmid and mix them well.
7. Load the sample and perform electroporation immediately to avoid cell sedimentation.
   Safety precautions: The metal part underneath the hinged cover and the metal part on the top of the inserted electroporation tube are high-voltage electrical connection terminals. Do not touch them with your hand. While the Celetrix electroporator is straighforward to operate, our electroporation tubes are specifically designed and they must be fully filled with a sample without air bubbles. For example, to load a 120 microliter tube , you'll need to take about 125 microliterl. Insert the pipette tip all the way into the electroporation tube. Rotate the tube and against the pipette tip to wet the bottom. Release the liquid steadily and keep the pipette tip end in the liquid while moving upwards until a bulging convex liquid surface is formed on the top. Press down the tube cover firmly with your thumb till you can feel the metal part on the top slightly sticking out.
   If there is a tiny bubble after loading, you can still continue as long as the diameter of the bubble is less than 1/3 of the tube.
   If there is an air bubble larger the 1/3 tube diameter, you'll need to empty the tube and reload it.
   When a sample is correctly loaded, you'll see a pulse record after electroporation.
8. Press the "Setting" icon to select parameter and then press "Done".
9. Press the "Push" button to release the hinged cover.
10. Insert an adapter into the socket and then insert the electroporation tube into the adapter.
11. Close the hinged cover and you'll see a blue light and the screen displays "GO".
12. Press the "GO" button to finish the electroporation and you'll see a pulse report immediately.
13. Remove the tube from the machine and transfer the cells to their culture medium.
14. Switch off the electroporator after the experiment is completed.

Model informations:

Product information: