MACSima™ Imaging Platform - Ultra high content imaging
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The MACSima™ Imaging Platform—Everything you need for fully automated, ultrahigh-content imaging
The possibility of evaluating localization, expression, and potential interaction of a multitude of different proteins allows scientists to tap the full potential of spatial biology.
Biological systems and cellular processes are inherently complex due to the interaction of thousands of proteins involved in proper functioning of the entire organism. Thus, an in-depth analysis of biological systems requires the examination of a plethora of parameters in order to decipher the underlying principles. Currently available techniques can provide only a very limited perspective on the complexity of biological systems.
MACSima™ Imaging Platform
With its automated processes and optimized components, the MACSima Imaging Platform reduces the effort required for the generation of complex high-content microscopy data to the basic essentials. The benefits of this complete, harmonized solution speak for themselves.
Hundreds of markers on one sample
Obtain high-content imaging data to analyze hundreds of proteins and other antigens on a single sample.
Fully automated instrument
Plan your experiment and leave the execution to the MACSima Imaging System.
Broad portfolio of validated antibodies
Analyze hundreds of markers on your sample based on a plethora of recombinantly engineered, fluorochrome-conjugated antibodies, validated specifically for MICS.
Effortless experiment preparation
Benefit from ready-to-use REAscreen™ Antibody Panels containing pre-defined sets of antibodies from Miltenyi Biotec for an effortless yet comprehensive analysis. Analyze any kind of fixed sample with our MACSwell™ Sample Carriers and benefit from various formats of antibodies for full flexibility.
Sophisticated yet simple analysis software
Analyze your ultrahigh-content imaging data easily and comprehensively using MACS® iQ View. Its versatility and intuitive user interface make this image analysis software the perfect companion for your spatial biology experiments.
Miltenyi Biotec’s MICS (MACSima™ Imaging Cyclic Staining) technology enables the simultaneous analysis of hundreds of markers on a single sample. Based on fluorescence microscopy, it uses the principle of cyclic staining with different fluorochrome-conjugated antibodies to acquire microscopy data for a multitude of parameters without harming the sample. The cyclic process comprises three main steps that are conducted by the MACSima Imaging System in a fully automated manner.
- Samples are stained with fluorochrome-conjugated antibodies
- Epifluorescence images are acquired of the desired sample areas
- The fluorescence signal is erased and the process restarts automatically
MACSwell™ Sample Carriers — Analyze any kind of fixed sample
MACSwell One, Two, and Four Imaging Frames can be mounted around any standard microscope slide and provide the reaction cavity needed for a MACSima Imaging experiment. MACSwell Imaging Frames are provided in three different sizes of reaction cavities to perfectly fit the size of your tissue sample.
MACSwell 24 Imaging Plates are pre-assembled and contain 24 rectangular wells with a clear 170 µm thick cover glass bottom. Simply pipet your cell suspension onto the plate, culture as usual and fix the sample according to your standard protocol directly on the plate.
MACSwell Micro Slides contain over 1.5 million hexagonal microcavities with a diameter of 20 µm and are therefore perfectly sized to fit exactly one cell. Simply mount your choice of MACSwell Imaging Frame around the slide and pipet your suspension cells in the resulting reaction cavity.
