MACS® Sample Preparation
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gentleMACS™ Tissue Dissociators and Tubes offer everything you need to reliably obtain viable single cells from any tissue. The combination of gentleMACS Tubes and Dissociators enables automated operation for standardized gentle dissociation or thorough homogenization on your benchtop. Start smart and preserve surface epitopes, as well as functionality of your cells of interest.
The gentleMACS™ Dissociators are a family of bench top instruments used for automated and standardized dissociation or homogenization of virtually any tissue. Viable single-cell suspensions are efficiently obtained by using our unique C Tubes in combination with our tissue-specific enzyme kits, whereas thorough homogenates are easily obtained by using the unique M Tubes. After processing, samples can be used for any cellular and molecular downstream analysis. Over 2,500 publications citing gentleMACS Technology proves that our devices can assist your research in multiple ways, such as
- generation of highly viable single-cell suspensions in a fast, standardized, automated, and user-independent way,
- ready-to-use tissue specific enzyme combinations with lot-to-lot consistency,
- optimized protocols preserve cellular composition and surface epitopes,
- single-use consumables minimize cross-contamination and allow easy disposal,
- closed and sterile system reduces handling hazards.
Keep it fresh – store tissue and keep its primary state
- The MACS® Tissue Storage Solution has been developed to allow an optimized storage of fresh organ and tissue samples without showing background effects like cell activation or apoptosis induction. It has been tested and validated on a variety of human and murine tissues including tumors, skin, heart, spleen, brain, and skeletal muscle.
Order no. Product Size 130-100-008 MACS Tissue Storage Solution 100 mL
• MACS Tissue Storage Solution represents a robust and convenient solution for short-term storage of different tissue samples and organs.
• Cell viability, cellular composition, and activation status of cells are well preserved for at least 48 hours.
• MACS Tissue Storage Solution prevents cellular stress responses, since changes in cellular stress-related gene expression levels were kept to a minimum compared to fresh sample material.
Be gentle – get viable cells with preserved epitopes
gentleMACS™ Technology delivers viable cells from solid tissues in a fast, standardized, and user-independent way, which preserves cellular composition and surface epitopes.
| Order no. | Product |
| 130-095-929 | Tumor Dissociation Kit, Human |
| 130-096-730 | Tumor Dissociation Kit, Mouse |
| 130-110-203 | Multi Tissue Dissociation Kit 2 |
| 130-098-373 | Neonatal Heart Dissociation Kit, mouse and rat |
| 130-105-807 | Liver Dissociation Kit, mouse |
| 130-095-927 | Lung Dissociation Kit, mouse |
| 130-105-737 | Umbilical Cord Dissociation Kit, human |
| 130-095-926 | Spleen Dissociation Kit, mouse |
| 130-097-410 | Lamina Propria Dissociation Kit, mouse |
| 130-092-628 | Neural Tissue Dissociation Kit (P) |
| 130-098-305 | Skeletal Muscle Dissociation Kit, mouse and rat |
| 130-101-540 | Whole Skin Dissociation Kit, human |
Clean it – remove cell aggregates and other unwanted material
Innovative cell strainers and cleaning reagents help you to tune your sample for your downstream application by the removal of cell aggregates, debris, or other unwanted material, such as myelin, dead cells, or erythrocytes.
gentleMACS™Technology
gentleMACS Technology allows fully automated tissue dissociation in a closed and sterile system to generate tissue lysates or viable single-cell suspensions with high viability and preserved surface epitopes. With the unique combination of alternating incubation time of enzymatic digestion and mechanical disruption, enzyme activity and shearing forces are lowered to a minimum. This makes gentleMACS Technology the most gentle and convenient method for standardized and reproducible tissue dissociation.
gentleMACS™ Dissociators—Automated tissue dissociation on your bench
The gentleMACS, gentleMACS Octo Dissociators and gentleMACS Octo Dissociator with Heaters offer reliable tissue dissociation and thorough homogenization with pre-defined programs and
parallel sample processing. The gentleMACS can process two samples in parallel with over 40 predefined programs. The gentleMACS Octo can process eight samples in parallel or independently and allows for the creation of user-defined programs. The gentleMACS Octo Dissociator with Heaters extends the basic features of the gentleMACS Octo Dissociator for maximum convenience, flexibility, and efficiency.
parallel sample processing. The gentleMACS can process two samples in parallel with over 40 predefined programs. The gentleMACS Octo can process eight samples in parallel or independently and allows for the creation of user-defined programs. The gentleMACS Octo Dissociator with Heaters extends the basic features of the gentleMACS Octo Dissociator for maximum convenience, flexibility, and efficiency.
| Order no. | Product | Size |
| 130-093-235 | gentleMACS Dissociator | 2 samples, semi-automated |
| 130-095-937 | gentleMACS Octo Dissociator | 8 samples, semi-automated |
| 130-096-427 | gentleMACS Octo Dissociator with Heaters | 8 samples with heater, fully-automated |
gentleMACS™ Tubes
The gentleMACS Tube is a central component of gentleMACS Technology. Each element of the tube has been engineered to ensure the highest performance in the dissociation or homogenization of your tissue samples. The cap has a rotor-stator-system to apply
gentle mechanical shearing to tissue.
Dedicated tubes for specific applications:
• Tissue dissociation: Use the purple-cap C Tube for gentle tissue disruption to get viable single-cell suspensions for cell separation, cell culture, and cell analysis experiments.
• Tissue homogenization: Choose the orange-cap M Tube to achieve thorough sample homogenization for subsequent molecular and
microbiology analysis
gentle mechanical shearing to tissue.
Dedicated tubes for specific applications:
• Tissue dissociation: Use the purple-cap C Tube for gentle tissue disruption to get viable single-cell suspensions for cell separation, cell culture, and cell analysis experiments.
• Tissue homogenization: Choose the orange-cap M Tube to achieve thorough sample homogenization for subsequent molecular and
microbiology analysis
| Order no. | Product |
| 130-093-237 | gentleMACS C Tubes (25×), sterile, single packed |
| 130-096-334 | gentleMACS C Tubes (100×), sterile, 25 tubes per bag |
| 130-093-236 | gentleMACS M Tubes (25×), sterile, single packed |
| 130-096-335 | gentleMACS M Tubes (100×), sterile, 25 tubes per bag |
| 130-094-392 | gentleMACS M Tubes with Strainer (50×), 50 tubes per bag with 600μm mesh strainer |
MACSmix™ Tube Rotator
The MACSmix Tube Rotator is a helpful tool for the enzymatic digestion steps during tissue dissociation with a gentleMACS Dissociator. It is a versatile instrument powered by rechargeable batteries operating independently of a permanent power supply. It is suitable for a temperature range of 2 °C to 42 °C and can be placed in a refrigerator or incubator.
| Order no. | Product | Size |
| 130-090-753 | MACSmix Tube Rotator | Tube rack for tubes from 0.5 mL to 50 mL in size |
Smart strainers and filters
MACS® SmartStrainers can be used for the removal of larger particles from cell suspensions of dissociated
tissue or blood samples:
MACS® SmartStrainers can be used for the removal of larger particles from cell suspensions of dissociated
tissue or blood samples:
- Improved ventilation during filtration avoids clogging of the strainers
- Easily fit onto standard 15 mL and 50 mL conical tubes
- Various mesh sizes are available, including 30, 70, and 100 µm to fit your specific application
| Strainer | Order no. | Size | Remarks |
| 30 μm (Yellow) | 130-098-458 | 50 filters | sterile, single-packed |
| 130-110-915 | 4 x 25 filters | sterile packed as 4x25 pieces | |
| 70 μm (Purple) | 130-098-462 | 50 filters | sterile, single-packed |
| 130-110-916 | 4 x 25 filters | sterile packed as 4x25 pieces | |
| 100 μm (Orange) | 130-098-463 | 50 filters | sterile, single-packed |
| 130-110-917 | 4 x 25 filters | sterile packed as 4x25 pieces |
Dead Cell Removal Kit
The Dead Cell Removal Kit is a fast and straightforward way of eliminating dead cells from cell cultures or tissue preparations. The Dead Cell Removal Kit contains ready-to-use MicroBeads and Binding Buffer for the magnetic labeling of cell debris, dead cells, and dying cells. The magnetically labeled material is removed by magnetic separation and pure, viable cells are obtained within 25 minutes.
The Dead Cell Removal Kit has been successfully used for the following applications:
Removal of dead cells prior to MACS® Separation of cells
- Reduction of flow sorting time by elimination of dead cells
- Elimination of dead cells from cell cultures
- Improvement of immunocytochemical analysis of frozen cells
- Preparation of viable, single-cell suspensions from tissue
- Removal of dead cells from spermatozoa samples
- Removal of dead cells prior to or following transfection of cells
| Order no. | Product | Size |
| 130-090-101 | MACS Dead Cell Removal Kit | for 1×10^9 total cells |
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